Virtual Conference
Shailaja Mane

Shailaja Mane

Dr. D Y Patil Medical College, Hospital and Research Centre, India

Title: Study of stem cells in human milk during transition of lactation


Stem cells are undifferentiated cells with ability to self-renewal. Each mammalian species has a unique composition of milk that has developed during evolution to fulfil the requirements of their new-borns and infants.  Breast milk contains stem and progenitor cells along with of immunological cells.

A Cross sectional, analytical, observational study was conducted after IEC approval. Healthy mothers delivered in the hospital were enrolled and breast milk samples were collected on day 1-2 (for colostrum), day 3-5 (for transitional milk) and day 6-7 (for matured milk). Isolation, detection  and differentiation of stem cells from preterm (<37 weeks),term (37 weeks to 41+6 days weeks) and post term milk (> 42 weeks) mothers was done. Total 30 mothers were enrolled by following inclusion and exclusion criteria of the study and written, informed consent.

Study revealed abundant number of stem cells. The stem cell count was highest on day 2 and it starts to decrease from day 3 to 7. The mean stem cell count in the human milk on day 3 was 3.18 *105 while on day 5 mean cell count was 1.28*105. Breast milk cells culture showed pluripotent mesenchymal stem cells with positive surface markers for CD 90, CD 105, and CD 73.  The mesenchymal stem cells of human milk further differentiated into Adipogenic, Chondrogenic and osteogenic lineages. Other parameters of mothers were also observed.

Conclusions: This study provides base line data of availability and types of stem cells in mothers milk with encouraging results highlighting encouraging future in the field of stem cells.

Implications: Breast milk can become a potential source of stem cells as its isolation remains a simpler and less invasive technique. It can open avenues for stem cell therapies and Regenerative medicine.


Shailaja Mane is a Pediatrician in Pimpri-Chinchwad, Pune and has an experience of 30 years in this field. Dr. Shailaja Mane practices at Siddhi Children's & Teen's Clinic in Pimpri-Chinchwad, Pune. She completed MBBS from Government Medical College, Miraj in 1991, MD - Pediatrics from Government Medical College, Miraj in 1993 and PGDPC (Post Graduation Diploma In Psychological Counselling) from Andhra Pradesh. She is a member of Indian Academy of Paediatrics (IAP), Indian Medical Association (IMA), National Neonatology Forum (NNF), IYCF, NHMB and AACCI. Some of the services provided by the doctor are: Nutritional Assessment, Vaccination/Immunization, Adolescent Medicine, New Born Care and Viral Fever Treatment etc.